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Object Flow Path & COPAS Sorting Principle
- Objects are passed axially, one by one, through the focus of a laser beam; the resulting signals are then detected and recorded by a forward scatter detector and fluorescence detectors.
- Size and optical density are simultaneously measured. Relative size is measured by an axial light-loss detector, which records the time that the light blockage signal remains above a pre-set threshold level (TOF). The optical density of the object is determined by the total integrated signal of the light blockage (EXT).
- The fluorescence intensity (FLU) is simultaneously determined at three different wavelengths by the excitation and emission filters in the system.
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